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KMID : 0545120120220091307
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 9 p.1307 ~ p.1309
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Soluble Expression of OmpA from Haemophilus parasuis in Escherichia coli and Its Protective Effects in the Mouse Model of Infection
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Ahn Jung-Oh
Hong Min-Hee
Yoo Sung-Sik
Lee Eung-Yo
Won Ho-Keun
Yoon In-Joong
Jung Joon-Ki
Lee Hong-Weon
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Abstract
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Haemophilus parasuis causes contagious porcine Glasser’s disease leading to severe losses in the swine industry. In this study, we established an efficient Escherichia colibased system for the expression of H. parasuis major outer-membrane protein (MOMP) that has been known as a good vaccine candidate against Glasser’s disease. Use of an E. coli-derived pelB leader sequence made it possible to produce recombinant MOMP (rMOMP) as the soluble forms without an additional refolding process. Using two different animal models, it was evaluated that the rMOMP was capable of inducing a significant immune response and providing protection against H. parasuis infection.
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KEYWORD
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Haemophilus parasuis, soluble expression, pelB leader sequence, antigen, Escherichia coli, OmpA
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